Wuhan Newcando Biotechnology Co.,Ltd

After the chest pain caused by myocardial injury, CK-MB began to rise within 3~8 hours in the blood and reached the peak concentration within 12 hours and recovered to the normal level within 36~72 hours. Therefore, CK-MB could be used as a marker for early diagnosis of acute myocardial infarction (AMI) and to provide rapid assistance for the diagnosis of AMI. When the coronary artery is blocked, the CK-MB in the cardiac myocytes will be washed out, resulting in the increase of CK-MB, which can be used to evaluate the effect of thrombolytic therapy according to the changes in the concentration of CK-MB.

The immunofluorescence double antibody sandwich method was used to detect the content of creatine kinase isoenzyme (CK-MB) in whole blood, serum and plasma samples. When the sample is added to the sample hole of the reagent card, if there is a CK-MB in the sample, the fluorescein particles with CK-MB antibody are combined with CK-MB to form a complex. The compound moves laterally along the test paper, and the CK-MB of the fluorescein particles is captured in the detection area, while the excess excess fluorescein particles are captured in the quality control area. When the reagent card was inserted into the Newcombe degree fluorescent immunoassay analyzer in Wuhan, the analyzer automatically scanned two ribbons and detected the fluorescence intensity of the compound emitted from the detection area and the quality control area. The content of the measured material was calculated by the ratio of two color band fluorescence intensity.

It is suitable for the fluorescent immunoassay quantitative analyzer produced by Newcombe bio Polytron Technologies Inc, Wuhan. Model: NCD-A.